Inhibition of P2X7 Receptor by Extracts of Chinese Medicine
Objective:To investigate the influence of Acorus gramineus(Soland),a crude extract,SCP01,and a purified component,SCP02,and of Rosmarinus officinalis L.,X0728 on human mast cells (HMC-1 Cell Line).Methods: Current-voltage of P2X7 receptors on human mast cell membrane activated by ATP was recorded by the whole-cell patch clamp technique.Results: The current at-100 mV mediated by P2X7 was inhibited by (27.6±2.0)％ in thepresence of 40μg/mL SCP01 and by (29.5±2.2)％ in the presence of 40μg/mL SCP02,which was identified as α-asarone.42μg/mL of the commercially available α-asarone inhibited the P2X7-mediated current by (52.2±2.0)％.In contrast to SCP01 and SCP02,40μg/mL X0728 provoked stimulation of the current by (28.6±2.8)％.All effects were voltage-independent.Conclusion:The inhibition of P2X7 by α-asarone will inhibit intracellular calcium increase and this may account for the inhibition of reported excitotoxic cell death.The pharmacological function of P2X7 stimulation by X0728 needs further investigation.
Purinoceptor P2X1 Acorus Tatarinowii Asarone Rosmarinus Patch-Clamp Technique
Andreas Spielmann GU Quan-bao MA Chung-hui HUANG Cheng-gang XUAN Li-jiang Wolfgang Schwarz
Max-Planck-Institute for Biophysics,Frankfurt/Main,Gernamy Shanghai Research Center of Acupuncture and Meridian,Shanghai 201203,P.R.China Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,P.R.China Max-Planck-Institute for Biophysics,Frankfurt/Main,Gernamy;Shanghai Research Center of Acupuncture a
国际会议
The 3rd Workshop on Scientific Approaches to Chinese Medicine(第三届中医科学研究方法研讨会)
安徽黄山
英文
286-288
2008-10-25（万方平台首次上网日期，不代表论文的发表时间）