RAPD and SCAR markers linked to a gene for stem nematode disease resistance in sweetpotato
The stem-nematode disease of sweetpotato is caused by Ditylenchus destructor and is a serious,widespread disease,causing tuber rot and severe losses in yield.The availability of DNA markers linked to genes for resistance to this disease would accelerate breeding programs.Bulked segregant analysis(BSA)was used to screen 280 random amplified polymorphic DNA(RAPD)primers for their ability to reveal polymorphism in 161 F1 populations derived from crosses between the resistant cultivars Xu 781 and the susceptible cultivars Xushu 18.1 RAPD markers were identified,OPD689,Iinked to the gene for stem nematode disease resistance.The distance between the RAPD marker and the stem nematode resistance gene in sweetpotato is 19.4 cM.The 689bp fragment of RAPD-OPD689 was cloned and RAPD marker was converted into a dominant sequence characterized amplified region(SCAR)marker,amplified a single fragment.This SCAR marker was identified in our research also tag this resistance gene and should be a valuable tool in facilitating marker-assisted selection for resistance breeding.
Sweetpotato Stem nematode disease resistance RAPD marker SCAR marker
Xin Wang Daifu Ma Qiang Li Yiping Xie
Xuzhou Sweetpotato Research Center,Xuzhou 221121,China
国际会议
3rd China-Japan-Korea Workshop on Sweetpotato(第三届中日韩甘薯学术研讨会)
北京
英文
352-356
2008-10-12（万方平台首次上网日期，不代表论文的发表时间）