Enhanced tolerance of transgenic sweetpotato plants overexpressing nucleoside diphosphate kinase 2 to environmental stresses
Oxidative stress is one of the important factors causing injury to plants exposed to environmental stresses.Nucleoside diphosphate kinase 2(NDPK2)is known to regulate the expression of antioxidant genes via hydrogen peroxide(H2O2)-dependent signal pathway in plant under stress conditions (Moon et al.,2003).In this study,we generated transgenic sweetpotato(Ipomoea batatas L.cv.Yulmi)plants expressing AtNDPK2 gene under the control of an oxidative stress-inducible sweetpotato peroxidase(SWPA2)promoter(Kim et al.,2003)(referred to as SN plants),and evaluated their tolerance to various environmental stresses.When leaf discs of SN plants were subjected to methyl viologen(MV),they showed less damage compared to non-transformed(NT)plants.Expression level of AtNDPK2 gene and NDPK activity in SN plants following MV treatment well reflected the plant phenotype.Interestingly,the activities of H2O2-scavenging enzymes such as peroxidase,ascorbate peroxidase and catalase were also increased in MV-treated SN plants.In addition,SN plants showed enhanced tolerance to chilling,high salinity and drought stresses.These results indicate that overexpression of AtNDPK2 under the control of SWPA2 promoter in sweetpotato might efficiently regulate the oxidative stress derived from various environmental stresses.
H2O2 scavenging enzyme Nucleoside diphosphate kinase 2 Oxidative stress Peroxidase promoter Sweetpotato
Yun-Hee Kim Hyun-Sook Woo Soon Lim Suk-Yoon Kwon Haeng-Soon Lee Dae-Jin Yun Sang-Soo Kwak
Environmental Biotechnology Research Center,Korea Research Institute of Bioscience and Biotechnology Plant Genomics Research Center,Korea Research Institute of Bioscience and Biotechnology(KRIBB),Daeje Division of Applied Life Science,Gyeongsang National University,Jinju 660-701,Korea
国际会议
3rd China-Japan-Korea Workshop on Sweetpotato(第三届中日韩甘薯学术研讨会)
北京
英文
329-331
2008-10-12（万方平台首次上网日期，不代表论文的发表时间）