Expression of an engineered tandem-repeat starch-binding domain in sweetpotato plant

摘要：

In this study,an artificial tandem repeat of a family 20 starch binding domain(SBD2) was engineered by two copies of the SBD derived from Bacillus circulans cyclodextrin glycosyltransferase via the Pro-thr-rich linker peptide from Xyn10A from Cellulomonas fimi.SBD2 was introduced into the sweetpotato Xu 55-2,using appropriate signal sequence.The presence of the SBD2 gene in the genomic DNA of transgenic sweetpotato plants was verified by PCR amplification as well as by Southern blot analysis.The accumulation of SBD2 into transgenic starch granules was confirmed by Western dot bolt analysis.Most striking were the observations that SBD2 expression could affect granule morphology without altering the primary structure of the constituent starch molecules.

关键词： Sweetpotato(Ipomoea batatas) Agrobacterium tumefaciens-mediated transformation Tandem starch-binding domain Starch affinity

作者: Yujun Xing Qin Ji Qing Yang Yuming Luo Qiang Li Xin Wang

作者单位: Department of Biology,Huaiyin Teachers College,Huaian,China;College of Life Sciences,Nanjing Agricul Department of Biology,Huaiyin Teachers College,Huaian,China College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,China Sweetpotato Research Institute,Chinese Academy of Agricultural Science,Xuzhou 221121,China

会议类型: 国际会议

会议名称: 3rd China-Japan-Korea Workshop on Sweetpotato(第三届中日韩甘薯学术研讨会)

会议地点: 北京

会议语种:英文

页码: 317-325

在线出版日期: 2008-10-12（万方平台首次上网日期，不代表论文的发表时间）