Transgenic sweetpotato plants expressing a cold-inducible zinc finger protein,SCOF-1
Sweetpotato Ipomoea batatas(L.) Lam. is one of the most important crops to produce food,feed and industrial materials. However,sweetpotato is sensitive to low temperature due to its origin from tropical and subtropical regions. SCOF-1 encodes a cold-inducible zinc finger protein in soybean, which functions as a positive regulator of cold-regulated gene expression(Kim et al. 2001). In this study, to develop the transgenic sweetpotato(cv. Yulmi) with enhanced tolerance to low temperature,we generated SCOF-1 expressing transgenic sweetpotato under the control of an oxidative stress-inducible SWPA2 promoter (Kim et al.2003) by Agrobacterium-mediated transformation. Hygromycin-resistant embryogenic calli were selected on selection medium and converted into plantlets which developed into morphologically normal plants. Putative transgenic plants were selected by PCR with hygromycin and SCOF-1 specific primers. The expression level of the SCOF-1 gene was confirmed by RT-PCR analysis in the leaves of transgenic sweetpotato plants. Further characterization of transgenic plants is under study response to cold stress.
Agrobacterium-mediated transformation Cold stress SCOF-1 Sweetpotato SWPA2 promoter
Myoung Duck Kim Kyoung-Sil Yang Hyun-Sook Woo Jung-Mae Liu Suk-Yoon Kwon Sang-Soo Kwak Haeng-Soon Lee
Environmental Biotechnology Research Center,Korea Research Institute of Bioscience and Biotechnology Plant Genomics Research Center,Korea Research Institute of Bioscience and Biotechnology(KRIBB),Daeje
国际会议
3rd China-Japan-Korea Workshop on Sweetpotato(第三届中日韩甘薯学术研讨会)
北京
英文
292-294
2008-10-12（万方平台首次上网日期，不代表论文的发表时间）