Transgenic sweetpotato plants expressing spike protein of porcine epidemic diarrhea virus

摘要：

Porcine epidemic diarrhea virus(PEDV) causes enteritis in swine of all ages,and is fatal in neonatal piglets.The spike protein of PEDV is a primary target antigen for developing an effective vaccine against coronaviruses,since it mediates essential biological functions.SweetpotatoIpomoea batatas(L.)Lam. is an attractive root plant producing plant-based edible vaccine as functional feed for swine.In this study,in order to develop the transgenic sweetpotato plants that produce an effective antigen protein against PEDV,we constructed the transformation vectors using partial fragment of PEDV spike protein(SP).Transgenic sweetpotato plants were successfully developed by Agrobacterium-mediated transformation and confirmed by PCR,Southern blot and RT-PCR analysis.

关键词： Agrobacterium-mediated transformation Edible vaccine Porcine epidemic diarrhea virus Sweetpotato

作者: Kyoung-Sil Yang Suk-Yoon Kwon Joon-Seol Lee Sang-Soo Kwak Haeng-Soon Lee

作者单位: Environmental Biotechnology Research Center,Korea Research Institute of Bioseience and Biotechnology Plant Genomic Research Center,Korea Research Institute of Bioseience and Biotechnology(KRIBB),Daejeo Mokpo Experiment Station,National Institute of Crop Science,Rural Development Administration,Muan 53

会议类型: 国际会议

会议名称: 3rd China-Japan-Korea Workshop on Sweetpotato(第三届中日韩甘薯学术研讨会)

会议地点: 北京

会议语种:英文

页码: 289-291

在线出版日期: 2008-10-12（万方平台首次上网日期，不代表论文的发表时间）