Protective role of hydrogen sulfide on chlorophyll degradation and antioxidant metabolism in seedling leaves of Ipomoea batatas exposure to osmotic stress
Protective role of NaHS,a hydrogen sulfide(H2S)donor on the content of chlorophyll and antioxidant metabolism in seedling leaves of sweetpotato under osmotic stress were investigated in this paper.The content of chlorophyll in seedling leaves of sweetpotato(Ipomoea batatas)decreased dramatically with the enhancement of osmotic stress,which was mimicked by PEG-6000.The chlorophyll content halved at 15％ PEG(w/v).The osmosis-induced chlorophyll loss could be alleviated by spraying with exogenous H2S donor,Naris in a dose-dependent manner,while little visible symptom was observed in leaves sprayed with Naris under normal condition.It was H2S or HS-rather than other sulfur-containing components derived from NaHS that attributed to the protective role in chlorophyll degradation against osmotic stress.Further studies showed that NaHS spraying dramatically promoted the activities of superoxide dismutase(SOD),catalase(CAT),ascorbate peroxidase(APX)and decreased that of lipoxygenase(LOX),whereas decreased the levels of hydrogen peroxide(H2O2) and malondialdehyde(MDA).In addition,higher level of endogenous H2S in NaHS-spraying seedlings was observed as comparison with water-spraying control.These data indicated that H2S was involved the antioxidant mechanism in sweetpotato seedlings’response to osmotic stress.
Antioxidant metabolism Chlorophyll degradation Hydrogen sulfide Osmotic stress Seedling leaves Sweet potato(Ipomoea batatas)
Hua Zhang Fang Wang Lijuan Bao Rui Wang Songhua Wang Jianping Luo
School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009 China Life Science College,Anhui Science and Technology University,Bengbu 233100,China
国际会议
3rd China-Japan-Korea Workshop on Sweetpotato(第三届中日韩甘薯学术研讨会)
北京
英文
178-186
2008-10-12（万方平台首次上网日期，不代表论文的发表时间）