Molecular characterization of bermudagrass germplasm using sequence‐related amplified polymorphism markers
Bermudagrass Cynodon dactylon ( L.) Pers. germplasm has a broad resource of genetic diversity that can be used for turfgrass,forage and conservation.bermudagrass is widely distributed in world, but genetical information is lacking on DNA molecular variation in geographically disparate forms.Accordingly, this study was conducted to assess molecular genetic variation and genetic relatedness among thirty‐five C.dactylon var.dactylon accessions and twenty‐one public cultivars originating from 4 countries ( China, Australia, USA and India).The objectives of this study were to use sequence‐related amplified polymorphism ( SRAP) markers in the evaluation of genetic diversity in a diverse collection of 56 bermudagrass germplasms.Thirty SRAP primer combinations were used.It is the first report of home and abroad bermudagrass germplasm resources relationship by using SRAP markers.Cluster analysis by the unweighted pair‐group method with arithmetic averages ( UPGMA) based on 268 (in total of 274) polymorphic bands indicated that there were six clusters (Figure 1).The coefficients of genetic distance among the genetypes ranged from 0.57 up to 0.97 and averaged 0.77.The genetic diversity estimate, He, average 0.27.Both Principal Coordinate Analysis (PCA) and UPGMA analysis indicated the similar results that the ecological varieties were clustered in different groups.The Australia cultivars, some USA cultivars, Xinjiang cultivars, and so on, were distinctly differentiated.This shows there is a wide genetic diversity among genotypes within C.dactylon.Ferriol et al.
SRAP Cynodon dactylon (L .) Pers . cultivars molecular characterization UPGMA
Z.Y.Wang J.X.Liu
Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing.210014,China
国际会议
呼和浩特
英文
2008-06-29（万方平台首次上网日期，不代表论文的发表时间）