Effect of different culture medium on subculture and differentiation of alfalfa anther callus
Cultivated alfalfa ( Medicago.sativ a.L ) is one of the high quality legume crop, which is wildly planted in the world.Alfalfa is cross‐pollination plant, genotype heterozygote and genetics diversity.Method of conventional breeding would not easy to obtain pure line.But pure line can be acquired by the use of anther culture to create double haploid (DH) lines in short time.Combined anther culture with cross‐breeding, heterosis utilization, transgenic technology have formed an effective breeding technology and become an important part of breeding.The effects of different medium components, hormone categories and concentrations was investigated in the experiment, to establish foundation for further optimize culture conditions and constructing doubled haploid population.
alfalfa anther callus tissue culture hormone macroelement
Li Zeng-hong Zhang Bo Chen Ai-ping Luo Yu-peng
Xinjiang Key Laboratory of Grassland Resources and Ecology,College of Grassland Science of Xinjiang Agricultural University,Urumqi,830052,China
国际会议
呼和浩特
英文
2008-06-29（万方平台首次上网日期，不代表论文的发表时间）