Effects of different enzyme combination and dissociation‐time on the protoplast isolation of alfalfa

摘要：

At present, as donor of protoplast dissociation is different to alfalfa, some factors have influence on protoplast dissociation, such as the kinds of enzyme, the concentration and dissociation‐time.Using hypocotyl of alfalfa as tested materials, by setting different the kinds of enzyme, the concentration and combination of enzyme, dissociation‐time in this study in order to provide theory and practical guidance for extension of alfalfa biotechnology.

关键词： alfalfa the kinds of enzyme concentration of enzyme dissociation‐time protoplast

作者: Chen Ai-ping Luo Yu-peng Yan Run Zhang Bo

作者单位: Key Laboratory of Grassland Resource and Ecology of Xinjiang,College of Pratacultural Engineering,XinjiangAgricultural University,Urumqi,830052,China.

会议类型: 国际会议

会议名称: 2008世界草地与草原大会

会议地点: 呼和浩特

会议语种:英文

在线出版日期: 2008-06-29（万方平台首次上网日期，不代表论文的发表时间）