Establishment and optimization of the ISSR‐PCR reaction system in Stipa krylovii
Species of Stipa L.of family gramineae is one of the most important forages on the grassland in north China.Stip a L.as the constructive species is dominant in the typical steppe zone of plant communities in Eurasian.Stipa krylov ii as a perennial grass of Stipa L.not only form the unique plant formation in steppe of middle Asia but also is dominant species in the main vegetation of Inner Mongolia plateau around Beijing (WANG Yanfang et al.,2006).Many studies of Stip a L.have mainly focused on their ecobiological characteristics and the impact of different ecological factors (eg.grazing gradient, fire, cutting) on the population ( AN Yuan et al., 2002 ).but there have been relatively few studies on genetic variability, particularly no papers on genetics Stipa krylov ii using ISSR molecular marker has not been published in China so far.The purpose of the paper is to establish and optimize ISSR‐PCR amplification sysytem.
Stip a krylovii ISSR reaction system optimization molecular marker
SUI Xiaoqing WANG Kun ZHENG Shuhua LIN Li
Institute of Grassland Science,College of Animal Science and Technology,China Agriculture University,Beijing 100094
国际会议
呼和浩特
英文
2008-06-29（万方平台首次上网日期，不代表论文的发表时间）