Application of ISSR‐PCR to identification two different ecotypes of Lespedeza hedysaroides

摘要：

Inter-simple sequence repeats-polymerase chain reaction (ISSR‐PCR) is a new type of molecular marker technology in recent years (Zietkiewiez, 1994).Universal primers were designed according to the genome of microsatellite sequences for the genomic DNA PCR amplification.ISSR‐PCR has been widely applied variety identification,fingerprint mapping and genetic diversity analysis.The objective of this research was to identify two different ecotypes of L.hedysaroides (the light green leaf and the dark green leaf) by Zhao Yang etc.(Zhao Yang, 2006) Lespedez a spp. ISSR‐PCR reaction system optimized.

关键词： Lespedez a hedysaroides ISSR-PCR molecular marker identification

作者: Yang Xiu-fang Sun Qi-zhong

作者单位: College of Grassland Science,Gansu Agriculture University,Lanzhou,Gansu Province 730070,China Grassland Research Institute,Inner Mongolia,Hohhot 010010,China

会议类型: 国际会议

会议名称: 2008世界草地与草原大会

会议地点: 呼和浩特

会议语种:英文

在线出版日期: 2008-06-29（万方平台首次上网日期，不代表论文的发表时间）