Genetic transformation of tomato pollen lysine‐rich gene to Elytrigia intermedium and regeneration of transgenic plants
Elytrigia intermedium (Hest) Nevski is a perennial Triticeae grass that has high biomass production,high palatability and digestion, but low lysine content.A pollen‐specific lysine‐rich gene cDNA sequence from tomato was obtained by RT‐PCR.A plant expression plasmid pBIUB‐TSB was constructed with the ubiquitin promoter.Plant regeneration was constructed using the mature seed of Elytrigia intermedia as the explant.Plasmids of pBIUB‐TSB were transferred into the calli by the particle gun and the laser microbeam methods.The regenerated plants were selected for kanamycin resistance.
Elytrigia intermedia lysine‐rich gene transformation regeneration
Xiaoping Lu Li Yin Fugui Mi Yuhong Xiao Shuyan Wang
College of Agron.,Inner Mongolia Agric.Univ.,Huhhot,Inner Mongolia 010018 P.R.of China Beijing Gen.Acad.in Sci.Acad.of China,Beijing 101300
国际会议
呼和浩特
英文
2008-06-29（万方平台首次上网日期，不代表论文的发表时间）