Drought‐tolerance in perennial ryegrass:a cisgenic approach
We employed a SAGETM ‐protocol using perennial ryegrass tissues sourced from active New Zealand pastures during the peak of autumn ; winter ; spring and summer seasons to understand the seasonal changes in gene expression.Analysis of 14,559 redundant SAGETM tags, which were present more than once in our SAGETM library, revealed season‐specific expression profiles for numerous genes that are likely to be involved in stress‐tolerance and plant‐growth and development ( Sathish et al.2007 ).We observed the ryegrass ortholog of Arabidopsis v acuolar py rophosphatase 1, which has been demonstrated to confer drought‐tolerance ( Gaxiola et al.2001 ), was expressed at least two‐fold greater in summer than in other seasons.We have decided to explore the options of enhancing this genes expression in cisgenic ryegrass plants to confer drought tolerance.
water‐deficit plant transformation dehydrin ( Dhn) promoter v acuolar py rophosphatase 1 (V P1)
K.Templeton C.Bryant M.Biswas G.Gill S.Bajaj P.Sathish Z.Hanley
Pastoral Genomics,c/o ViaLactia Biosciences,PO Box 109185,Newmarket,Auckland,New Zealand.
国际会议
呼和浩特
英文
2008-06-29（万方平台首次上网日期，不代表论文的发表时间）