Establishing an abundant and efficient explant system for the Agrobacterium‐mediated transformation of zoysiagrass
Zoysiagrass ( Zoysia j aponica Steud.) is one of the most important warm season turf grass species native to the Far East.This turf grass is distinguished by a low nutrient requirement, and tolerance to environmental stresses such as drought and salinity.However, thedifficult seed germination and callus production, has seriously blocked attempts to perform biotechnology on the species.Our aim has been to develop a system for regeneration from embryo drived callus of zoyiagrass that can be used for A grobacterium ‐mediated transformation with genes to improve cold tolerance.. To ensure a abundant and constant supply of explantsmature seeds and embryos isolated from mature seeds were used for callus induction..
explants callus transformation embryo zoysiagrass
X.Wang Y.Hoshino T.Yamada
Graduate School of Environmental Science,Hokkaido University,Kita 11,Nishi 10,Kita,Sapporo 060-0811, Field Science Center for Northern Biosphere,Hokkaido University,Sapporo 060-0811,Japan
国际会议
呼和浩特
英文
2008-06-29（万方平台首次上网日期，不代表论文的发表时间）