2-Hydroxy-4-methoxybenzophenone (HMB) and 2,4,4-trihydroxybenzophenone (THB) Suppress Amphibian Metamorphosis

摘要：

Widely used in cosmetics and plastics, the class of chemical substances known as chemical ultraviolet (UV) filters make their way into the environment, polluting rivers and lakes and eventually accumulating in tissues of wildlife and humans. The ultimate consequence of this is unknown, although an increasing number of studies have confirmed the estrogenic activities of many of these substances. In the present study we make use of T3 induced enhancement of amphibian metamorphosis to screen HMB and THB, two commonly used UV filters, for possible effect on thyroid hormone (TH) related processes. Our findings show that both substances inhibit T3-induced tail atrophy and apoptosis in Rana rugosa tadpoles, as well as suppress spontaneous metamorphosis in Silurana tropicalis. Using transgenic Xenopus laevis tadpoles expressing EGFP driven by TR β A1 promoter region containing TH response element (TRE), these chemicals were found to prevent the binding of T3 to TH receptor (TR), suggesting that both HMB and THB act as T3 antagonists.

关键词： amphibian metamorphosis apoptosis endocrine disruptors HMB and THB TH antagonist T3 induced metamorphosis transgenic frog UV filters

作者: KASHIWAGI Keiko TOOI Osamu SUZUKI Ken-ichi YOSHIZATO Katsutoshi KASHIWAGI Akihiko HANADA Hideki YAMAMOTO Takeshi GOTO Yasushi FURUNO Nobuaki KITAMURA Shigeyuki OHTA Shigeru SUGIHARA Kazumi TANIGUCHI Kikuyo

作者单位: Institute for Amphibian Biology,Graduate School of Science,Hiroshima University,1-3-1 Kagamiyama,Hig Institute of Environmental Ecology,1DEA Consultants,Inc.,1334-5 Riemon,Ooigawa,Shida,Shizuoka 421-02 Laboratory of Developmental Biology,Department of Biological Science,Graduate School of Science,Hiro ProPhoenix Company,3-13-26,Kagamiyama,Higashihiroshima 739-0046,Japan Institute for Amphibian Biology,Graduate School of Science,Hiroshima University,1-3-1Kagamiyama,Higa Hiroshima Prefectural Police,9-42 Hon-machi,Naka-ku,Hiroshima 730-8507,Japan Nihon Pharmaceutical University,10281 Komura,Ina-machi,Kitaadachi-gun,Saitama 362-0806,Japan Graduate School of Biomedical Sciences,Hiroshima University,Kasumi1-2-3,Minami-ku,Hiroshima 734-8553 Graduate School of Biomedical Sciences,Hiroshima University,Kasumi 1-2-3,Minami-ku,Hiroshima 734-855 School of Medical Technology,Sanyo Womens College,Sakatahonmachi 1-1,Hatsukaichi 738-8504,Japan

会议类型: 国际会议

会议名称: The 2008 International Symposium on Safety Science and Technology(2008年安全科学技术国际会议)

会议地点: 北京

会议语种:英文

页码: 970-976

在线出版日期: 2008-09-24（万方平台首次上网日期，不代表论文的发表时间）