Screening of PAH-Degrading bacteria and studies on their degradation characters
PAH-degrading microbes CNWL2 and CNWL6 were isolated from bottom sludge of coking wastcwater.By using the technique of bacterial adherence to hydrocarbons(BATH),cell-surface properties of the two strains had been investigated,and by using shaking flask test,MPN method and UV Spectrophotometric Method.Their degradation properties for pyrene and the influence of some metabolic intermediates on the degradations had also been studied.The results indicated that cell surface of CNWL2 is with strong hydrophobicity and that Of CNWL6 is with sffong hydrophilicity.Both CNWI.2 and CNWL6 could decompose pyrene and the biodegradation rate was higher bv CNWL2 than by CNWL6.For CNWL2.The pyrene degradarion rate can be enhanced 25％and 16％by adding phthalic acid and glucose respectively.CNWL6,with higher hydrophilicity,displayed high adsorptivity to pyrene,and its pyrene-degrading process could be inhibited by adding phthalic acid and glucose in cultural liquid.The pH values of biodegrading medium were fluctuating up and down continuously.This pH value fluctuations were resulted from accumulating of acidic metaboric intermediates and further decarboxylation of such intermediates.
predominant strains biodegradation pyrene metabolic intermediates
WANG Lei NIE Mai-qian WANG Zhi-ying WU Man-li
School of Environmental and Municipal Engineering,Xian University of Architecture and Technology,Xian,710055,Shaanxi Province,China
国际会议
西安
英文
573-580
2008-05-15（万方平台首次上网日期，不代表论文的发表时间）