Physicochemical and Transfection Properties of Quaternized Cellulose synthesized in NaOH/Urea Aqueous Solutions
Quaternized celluloses (QCs) were homogeneously synthesized by reacting cellulose with 3-chloro-2-hydroxypropyltrimethylammonium chloride (CHPTAC) in NaOH/urea aqueous solutions. The structure and solution properties of the QCs were characterized by using elemental analysis, FTIR, 13C NMR, SEC-LLS, viscometer and zeta-potential measurement. The results revealed that water-soluble QCs with degree of substitution (DS) value of 0.20 to 0.63 could be obtained by adjusting the molar ratio of CHPTAC to anhydroglucose unit (AGU) of cellulose and the reaction time. The QC solutions in water displayed a typical polyelectrolyte behavior, and the intrinsic viscosity (?) value determined from Fuoss-Strauss method increased with increasing DS value. Moreover, two QC samples (DS=0.46 and 0.63) were selected and studied as gene carriers. The results of gel retardation assay suggested that QCs could condense DNA efficiently. QCs displayed relatively lower cytotoxicity as compared with PEI, and QC/DNA complexes exhibited effective transfection in compared to the naked DNA in 293T cells. The quaternized cellulose derivatives prepared in NaOH/urea aqueous solutions could be considered as promising non-viral gene carriers.
Yongbo Song Yunxia Sun Xianzheng Zhang Jinping Zhou Lina Zhang
Department of Chemistry,Wuhan University,Wuhan 430072,China
国际会议
5th International Symposium on High-Tech Polymer Materials(HTPM-V)(第五届国际高技术高分子材料学术会议)
北京
英文
174
2008-10-27（万方平台首次上网日期，不代表论文的发表时间）