Bio-fabrication and Functionalization of Cellulose Nano-fibers Composites
(0) For the multi-functional materials with unique architectures and defined patterns, the key point is how to design and control the fine structures from the molecular to the nano-and microscales. Bacterial cellulose (BC),produced by Acetobacter xylinum, consists of ribbon-shaped nanofibers in weblike structures. BC can form favored materials with high mechanical strength in wet state and enormous water retention values. Furthermore,BC has the property of substantial permeability for liquids and gases and the moldability during cultivation process. Therefore, various functional materials can be obtained by blending BC with inorganic or organic molecules.In this paper, a new approach for controllable bio-fabrication of patterened cellulose nano-fibers has been proposed by micro-fluidic techniques, via the combination of biological technology and nanotechnology. We attempted to make sure whether A. Xylinum can regularly move within micro-fluidic channels and further to clarify how the flow can direct and control the assembly of cellulose nano-fibers. The well-patterned materials have great potential utility in tissue engineering and opto-electrical materials.Furthermore, the novel artificial skin of bacterial cellulose have been biosynthesized by ,4cetobacter xylinum and modified by chitosan or collagen. NIH/3T3 cell strain is used for in vitro biological evaluation. The biocompatibility of the films was evaluated by MTT assay, cell proliferation assay and Cell adhesion. The results demonstrated the composites films have potentials for novel artificial skin. The nano-cellulose /chitosan or collagen composites exhibit good performances as new skin substitutes and modern wound dressing materials using tissue engineering which are the prospect of application and very wide clinical value.Moreover, three kinds of electronic materials (carbon nano-tube, tetraethoxysilane and polyaniline) have been respectively in situ assembled into the ordered 3D cellulose network of nanofibers during or after the cultivation. The morphology, supermolecular structure and conducting properties of the composites were characterized by Fourier transform Infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Environmental Scanning Electron Microscopy (ESEM), the measurement for the resistance and dielectric constant. The results indicated that electronic molecules could be assembled into the 3D cellulose network of nanofibers, the conducting ability of the composites were significantly improved.
bio-fabrication bacterial cellulose micro-fluidic technique artificial skin opto-electrical materials
Guang Yang Xudian Shi Gang Wang Lina Fu Zhijun Shi
College of Life Science & Technology,Huazhong University Science & Technology,Wuhan,430074
国际会议
5th International Symposium on High-Tech Polymer Materials(HTPM-V)(第五届国际高技术高分子材料学术会议)
北京
英文
35
2008-10-27（万方平台首次上网日期，不代表论文的发表时间）