Cultivation and properties of endothelial cells on anionic gel scaffolds
1. Introduction Hydrogels are appealing materials for tissue engineering due to their structural similarity to macromolecular-based extracellular matrix in biological tissue. However, there are significant biological and physical shortages of synthetic hydrogels, such as poor cellular viability and weak mechanical strength, greatly prevent the wide application of synthetic hydrogels in tissue engineering field. In physiological condition, the role of tissue cells is important in biological activity. For examples, a continuous endothelial cell (EC) monolayer at the inner surface of blood vessel contributes to protecting procoagulant activity. Needless to say.Many tissues, such as blood vessel exist in a severe mechanical dynamic environment. Therefore, from a potentially practical view point, in order to replace the natural tissues with hydrogels, design and production of hydrogels with some critical parameters, such as high cellular viability and mechanical strength, are crucially important in the biomedical applications of hydrogels. We have strived to develop hydrogels with these critical parameters by using synthetic polymers besides natural polymers. The following topics have been investigated:1) develop adhesive protein free synthetic polymer gels as scaffolds for cell cultivation,2,3,2) control cell functions by gel properties4,and 3) creation tough hydrogels for cell cultivation5.We believe these studies would substantially promote the application of hydorgels for tissue engineering.
Yong Mei Chen Yoshihito Osada Jian Ping Gong
Graduate School of Science,Hokkaido University,Sapporo 060-0810,Japan;School of Science,Xian Jiaoto Graduate School of Science,Hokkaido University,Sapporo 060-0810,Japan;Present address: RIKEN 2-1,Hir Graduate School of Science,Hokkaido University,Sapporo 060-0810,Japan
国际会议
5th International Symposium on High-Tech Polymer Materials(HTPM-V)(第五届国际高技术高分子材料学术会议)
北京
英文
13-14
2008-10-27（万方平台首次上网日期，不代表论文的发表时间）