In Vitro Evaluation of a 3D PLGA-TCP Composite Scaffold in an Experimental Bioreactor
The development of a technique that enables the formation of three-dimensional (3D) cell/biomaterial complex architectures in a designed pattern is the basis for organ manufacturing. The scaffold attempts to mimic the structure and functions of extracellular matrix. These 3D living tissue/organ scaffolds/analogies can be used for in vitro experiments. The scaffold serves as an adhesion surface for the cells, and provides temporary mechanical support for the designed tissues or organs. We tested a 3D PLGA-TCP (poly-lactic acid-co-glycolic acid/tricalcium phosphate) composite scaffold. The scaffold was generated with the low-temperature deposition modelling (LDM) RP technique. The aims were to find optimal cell culture conditions for the selected scaffold material and to monitor cell division, differentiation and migration of selected cell types in this environment. Our further aims were to monitor behaviour and cell-matrix interactions of selected cell types and to monitor the biodegradation rate of the tested scaffold material. We used chinese hamster ovary (CHO) cells as well as a human cell line 293 epithelial cells and cultured them on top of the scaffolds. We also used a variety of different preconditions to prepare the scaffolds before the cultivations as well as various inoculations (on top of the scaffold or into it) to seed the cells. Cell cultivations were conducted for 1 to 4 weeks and the coverage of the luminal surfaces were analysed with light microscopy. Long cultivation periods were required to achieve partial coverage of the luminal surfaces of the scaffolds. Tissue engineering with 3D cell cultures and biomaterials represents a promising approach for organ manufacturing research. It may have potential for eventual on-demand high-throughput production of artificial tissues but the process has many challenges.
rapid prototyping, scaffold tissue culture biomanufacturing
Antti A. M(a)kitie Yongnian Yan Kaija-Stiina Paloheimo Xiaohong Wang Zhuo Xiong Jukka Tuomi Jari Salo Markku Paloheimo Risto Renkonen
Helsinki University of Technology, Espoo, Finland;Dept. of Otolaryngology, Helsinki University Hospi Dept. of Mechanical Engineering, University of Tsinghua, Beijing, China Helsinki University of Technology, Espoo, Finland Dept. of Orthopaedics and Traumatology, Helsinki University Hospital, Helsinki, Finland Haartman Institute, University of Helsinki, Helsinki, Finland
国际会议
北京
英文
153-157
2008-11-06（万方平台首次上网日期，不代表论文的发表时间）