Design and study of cell encapsulator for cell assembly technology
A cell encapsulation device based on the principle of high voltage dispersion technology was designed and manufactured. Sodium alginate was solidified in calcium chloride solution. Parameters influencing the forming of cell microencapsules like voltage, push speed, electrode distance and syringe inner meter were analyzed and optimized. Unlike the traditional cell encapsulator, both the electrodes were separated from calcium chloride solution in this device, which simplified the operation and provided a sanitary enviorment. Integrated adipose derived stem cells (ADSCs) microencapsules with smooth surface and even diameter distribution were prepared via the device. After one-week-long culture as microencapsulation and 3 days assembly in a construct, ADSCs maintained high viability and demonstrated significant proliferation, which established a favorable basis for the controlled assembly of cell microencapsules.
controlled assembly high voltage dispersion technology microencapsulation adipose derived stem cells (ADSCs)
Rui Yao Renji Zhang Xiaohong Wang
Key Laboratory for Advanced Materials Processing Technology, Ministry of Education & Center of Organ Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084,P.R. China;Institute of Life Science & Medicine, Tsinghua Univers
国际会议
北京
英文
112-121
2008-11-06（万方平台首次上网日期，不代表论文的发表时间）