Experimental Intervertebral Spinal Fusion in Goats with a RP-based PLGA/TCP/bBMP Biomimetic Grafting Material
Three-dimensional highly porous structural Poly (DL-lactic-co-glycolic acid)/Tricalcium phosphate (PLGA/TCP) scaffolds (10mm in diameter and 10 in height) were synthesized via a rapid prototyping (RP) technique. Bovine bone morphogenetic protein (bBMP) was loaded into the biopolymer scaffolds (PLGA/TCP/bBMP). Both the PLGA/TCP scaffolds and the PLGA/TCP-bBMP composites were observed by scanning electron microscopy. Lumbar intervertebral body fusion at L2~3 and L4~5 levels were performed on fifteen goats using one of the following graft materials: RP synthesized PLGA/TCP scaffolds (group A, n=10); PLGA/TCP-bBMP composites (group B, n=10); and autogenous iliac bone graft (group C, n=10). The animals in each group were killed twenty-four weeks after surgery and the spine fusion were evaluated by a general observation, a manual palpation test, histological analyses and radiography. As a result, RP established PLGA/TCP scaffolds with good biomaterial properties including satisfactory microstructure and porosities. bBMP was efficiently loaded on the highly porous structral scaffolds to produce a new biomimetic artificial grafting material. In group A, histological analyses showed PLGA/TCP scaffolds were biocompatible and biodegradable. Whereas, no newly formed bone was foung.In group B, highly cellular bone marrow between the newly formed trabecular bone were present in the fusion mass. In group C, there was a reduced amount of newly formed bone. Twenty-four weeks after surgery, the fusion rate of lumbar intervertebral body fusion in group A, B and C was 10% (1/10), 80% (8/10) and 50% (5/10), respectively. The fusion rate was significantly higher in group B compared with groups of A and C (P<0.01). Therefore, extracted bovine bone morphogenetic protein can be in vitro efficiently loaded into RP-based highly porous structural PLGA/TCP scaffolds to fabricate a new graft material for spinal fusion. RP-based PLGA/TCP/bBMP composites appear to be more effective for intervertebral spinal fusion. The biomimetic artificial grafting material is a promising novel tool for spine surgery. Further studies are required to confirm the medium- to long-term results.
rapid prototyping (RP) scaffold bone morphogenetic protein (BMP) spine fusion goat
Xing Ma Xiaoming Wu Yunyu Hu Zhou Xiong Rong Lu Jun Wang Dan Li Yongnian Yan
Institute of Orthopaedic Surgery & Department of Orthopaedics, Xijing Hospital, The Fourth Military Department of Biomedical Engineering, The Fourth Military Medical University, Xian 710032, Peoples Department of Mechanical Engineering, Tsinghua University, Beijing 100084, Peoples Republic of Chin
国际会议
北京
英文
85-90
2008-11-06（万方平台首次上网日期，不代表论文的发表时间）