To fabricate gradient hydrogel scaffold based on an improved cell assembling system
To manufacture complicated human organs in vitro, it is needed of the fabricating technology for gradient hydrogel scaffold based on rapid prototyping principle. Hydrogel material can provide biocompatible and biodegradable bionic extracellular matrix environment for cell survival. First we proposed a two-step crosslinking method that not only was suitable for forming process, but also caused little harm to cells. Then we developed a forming system which was derived from commercial rapid prototyping equipment. This system contained two types of nozzle units. One was double-nozzle unit,used for relatively simple gradient hydrogel scaffold composed of two kinds of nonmiscible hydrogel material units. The other was material-mixing single-nozzle unit, used for relatively complicated gradient hydrogel scaffold composed of continuous gradient by mixing two kinds of hydrogel material units.Different types of gradient scaffolds could be formed by selecting one relevant type of nozzle unit. To improve the extrusion controlling effect, some accessorial forming parameters were introduced.Experiments proved that the forming accuracy was improved, and both of the two types of nozzle units could work effectively. And at last, a 3D gradient scaffold containing neuron cells and Schwann cells was successfully fabricated, and the scaffold was cultured for 7 days. Though nerve fibers were not observed,this scaffold was a helpful example for later design of gradient scaffold to study the relations between different cells in vitro. All of these works were elementary for later manufacturing of complicated organ analogs, what might be a meaningful improvement for tissue engineering.
rapid prototyping tissue engineering biomanufacturing hydrogel scaffold cell assembling neuron cell Schwann cell
Shengjie Li Yongnian Yan Zhuo Xiong Chenyang Weng Xiaohong Wang Renji Zhang
Department of Mechanical Engineering, Tsinghua University, Key Laboratory for Advanced Materials Processing Technology Ministry of Education, P.R.China, Beijing 100084
国际会议
北京
英文
75-84
2008-11-06（万方平台首次上网日期，不代表论文的发表时间）