The scaffold with a branched channel system fabricated by SFF to favor a novel device for timely cell observation
A β-TCP scaffold with a branched channel system has been designed to favor a novel microdevice that allowed perfusion culture and direct observation timely of cells attached. The scaffold was made by indirect solid free form fabrication (SFF). The flow channel structure was exposed and could view in a direct view. Negative model of scaffold was designed by CAD software and its epoxy resin mold was fabricated on a sterolithography (SL) apparatus and the β-TCP slurry was filled in these molds. After β-TCP was self solidified, the mold was baked. The mold was removed by pyrolysis and then the designed scaffold was obtained. The perfusion culture of the mesenchymal stem cells (MSCs) were labeled with DiI. Then the cell seeded scaffolds were continuously perfused for 7 days in the microdevice.It was shown from the experiment of dynamic culture that cells were well adhered to the scaffolds and cell growth could be observed directly and timely. Cell distribution within the scaffold was different for the nutrients supply and fluid shear stresses are different. It was concluded that the device and the fabricated scaffold could allow the continuous and dynamic observe in a large view during the culture of cells and could be used to study the effect of fluid shear stresses on cell proliferation in a direct view. The basic design concept presented showed great promise for use in bone tissue engineering and fabrication method enhanced the versatility of scaffold fabrication.
Scaffolds Rapid prototyping Cell Flow channel
Shanglong Xu Yang Yue Chaofeng Wang Zhen Wang
School of Mechatronics Engineering, University of Electronic Science and Technology, Chengdu, China School of Mechanical & Vehicle Engineering, Beijing Institute of Technology, Beijing, China Xijing Hospital, The Fourth Military Medical University, Xian, Peoples Republic of China
国际会议
北京
英文
44-51
2008-11-06（万方平台首次上网日期，不代表论文的发表时间）