Fabrication and in vitro evaluation of calcium phosphate cement combined with controlled chitosan-fiber structure
The fiber-reinforced organic/inorganic composites, which combine the flexibility and bio-resorbability of an organic polymer with the stiffness, strength and osteoconductivity of a ceramic,have been increasingly investigated for their using in load-bearing situations as bone substitutes or in tissue engineering scaffolds. They have a nature of gradient degradability so that the fibers firstly dissolved to leave cylindrical macropores inside the scaffolds for bone growth, which further permit load transfer from the scaffolds to the host bone. A rapid prototyping and rapid tool technique (RP/RT) based method was previously developed to fabricate chitosan-fiber calcium phosphate cement composite (CF/CPC) for bone tissue engineering scaffold application. This study characterized its solidification products and the in vitro performance of canine bone marrow stem cells (BMCs) on CF/CPC scaffold with controlled fiber structures. X-ray diffraction (XRD) results showed that about 91% inorganic part of CF/CPC scaffold was hydroxyapatite (HA), whose content was irrelative with the fiber structure. The results from in vitro study demonstrated that the interconnected macropores could rapidly be formed inside the CF/CPC scaffolds and their patterns were relative with their fiber structures, but the difference of the fiber structures could alter the morphology of the BMCs without affecting the proliferation of the BMCs.
fiber-reinforced composite scaffold fiber structure biocompatibility
Qin Lian Dichen Li Zhongmin Jin Jue Wang AIMIN LI ZHEN WANG ZHONGMIN JIN
State Key Laboratory for Manufacturing Systems Engineering, Xian Jiaotong University, Xian 710049, Institute of Orthopedic Surgery, The Fourth Military Medical University, Xian 710032, China Institute of Medical and Biological Engineering, School of Mechanical Engineering, University of Lee
国际会议
北京
英文
10-15
2008-11-06（万方平台首次上网日期，不代表论文的发表时间）