Influence of lactic acid on the proliferation, metabolism, and osteogenic differentiation of mesenchymal stem cells
Mesenchymal stem cells (MSCs) may face a poor environment in high lactic acid originated from cell cultures, degradation of biomaterials, and other sources. This study aimed to determine if proliferation, metabolism, and osteogenic differentiation of MSCs were influenced by decreasing pH, increasing osmotic pressure, or chemical action of lactate ion. It was seen that the proliferation and metabolism of MSCs was inhibited in cultures with decreasing pH or high lactate.However, when osmolarity was adjusted to the same condition as that of sodium lactate using sodium chloride, it did not show the same degree of cell proliferation and metabolism influence. We also concluded that colony-forming efficiency and osteogenic differentiation were significantly depressed by low pH or high lactate. As was shown, the inhibition of lactate was not only due to osmotic pressure, but also mainly due to chemical effect of lactate ion. Thus, pH control is necessary, but not sufficient, to eliminate the influence of lactic acid on losing stem-cell characteristics of MSCs.
colony-forming units assays lactic acid mesenchymal stem cells differentiation proliferation.
国际会议
The 12th Asian Pacific Confederation of Chemical Engineering Congress(第十二届亚太化工联盟大会暨化工展览会)
大连
英文
2008-08-04（万方平台首次上网日期，不代表论文的发表时间）