E. coli-catalyzed Chemo-selectively Reduction of 4-Nitrophthalimide to Hydroxyl Deriveative
4-Nitrophthalimide was chemo-selectively reduced into its corresponding hydroxylamine catalyzed by E. coli cell extracts. This biocatalysts lost its activity after being boiled or the addition of specific nitroreductase inhibitors, indicating that nitroreductase might be involved. Kinetics analysis demonstrated that this reduction was a first order reaction with the rate constant k of 0.2292h -1 and the half life t1/2 of 3.024h. Surprisingly, this reaction had very limited substrates, which means it could only take 4-nitrophthalimide as substrate, but could not recognize other aromatic nitro group, such as 3-nitrophthalimide, 3,5-dinitrobenzoic acid,2-nitrotoluene,4-nitrotoluene and nitrobenzene. This selectivity might be due to the electron effects and regio-structure effects. The phenomenon might be helpful in organic synthesis of particular compounds.
E. coli chemoselecitivity 4-nitrophthalimide arylhydroxylamine
Bo Xie Qing Yang Xuhong Qian Jun Yang
Department of Bioscience and Bioengineering, Dalian University of Technology,Dalian, China Shanghai Key Laboratory of Chemical Biology, East China University of Science and Technology, Shangh
国际会议
The 12th Asian Pacific Confederation of Chemical Engineering Congress(第十二届亚太化工联盟大会暨化工展览会)
大连
英文
2008-08-04（万方平台首次上网日期，不代表论文的发表时间）