Numerical Simulation of Aggregation of cell-microbeads

摘要：

The aggregation of microtissue has great influence on the viability of internal cells and the in vitro fabrication of large-scale engineered tissue. In this article, we investigated the effect of cell seeding density, microcarrier concentration and Vc on the formation of microtissue and the size of aggregates in microcarrier culture system in spinner flask. We also developed a mathematical model about microtissue formation and aggregates size according to that. The results showed that the process of aggregation had three phases: lag phase, logarithmic phase and static phase. The average aggregate size of microtissue increased with the increase of cell seeding density and microcarrier concentration and the addition of Vc. Our mathematical model can successfully simulate the microtissue formation process and the aggregates size under different operation parameters.

关键词： tissue engineering microtissue aggregation mathematical model

作者: Mei Yang Zhou Yan Tan Wen-song

作者单位: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , Shanghai 200237, China

会议类型: 国际会议

会议名称: The 12th Asian Pacific Confederation of Chemical Engineering Congress(第十二届亚太化工联盟大会暨化工展览会)

会议地点: 大连

会议语种:英文

在线出版日期: 2008-08-04（万方平台首次上网日期，不代表论文的发表时间）