Feasibility and strategy of high efficiency fermentative H2 production from corn stalk liginocellulose

摘要：

In order to investigate the posibility of a pretreatment-delignification ?usimultaneous saccharification and fermentation (SSF) strategy for bio-hydrogen from cellulosic feedstock, batch tests were conducted using co-culture of mesophilic isolates, Clostridium acetobutylicum X9 (could effectively hydrolyze the partially delignified lignocellulose complex) and Ethanoigenens harbinense B49 (highly hydrogen production bacterium). The results showed that for the pretreatment-delignification step, acidic stream explosion (1% w/v H2SO4, 121 ℃, 15 psi for 2h) was applied to pretreat the corn stalk, and the residue following steam explosion was washed by water to separate the lignocellulose solid. For the SSF step, C. acetobutylicum X9 and E. harbinense B49 were inoculated, and simultaneous the resulting hydrolysate was used as the carbon substrate for initial pH of 7.0 for 30 h. With an initial corn stalk concentration of 15 g/l, the H2 production and yield was approximately 458.0 ml/l and 6.3 mmol H2/g cellulose, and cellulose degradation ratio of 63.3% were obtained respectively. This work implied the feasibility of a delignification→usimultaneous saccharification and fermentation process for hydrogen production from corn stalk liginocellulose.

关键词： biohydrogen simultaneous saccharification and fermentation (SSF) strategy process liginocellulose

作者: WANG Aijie GAO Lingfang REN Nanqi

作者单位: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, China; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, China

会议类型: 国际会议

会议名称: The 12th Asian Pacific Confederation of Chemical Engineering Congress(第十二届亚太化工联盟大会暨化工展览会)

会议地点: 大连

会议语种:英文

在线出版日期: 2008-08-04（万方平台首次上网日期，不代表论文的发表时间）