Optimizing Assay Conditions of Chalcone Isomerase Activity in Ginkgo biloba by Quadratic Orthogonal Rotation Design
By the experiment of quadratic orthogonal rotation combination design,the influence of different assay conditions on the activity of chalcone isomerase (Y) in Ginkgo biloba has been probed into in this paper,they include reaction temperature (X1),pH value (X2),reaction time (X3),substrate concentration (X4).The mathematical model of the CHI activity has been established which are influenced by these determination conditions: Y = 55.6167-0.7283X1-1.7258X2-0.3633X3-0.6217X4-2.3521X12-1.7908X22-0.9783X32-0.7121X42-0.0125X1X2-0.0088X1X3-0.0800X1X4+0.3238X2X3-0.1225X2X4-0.0813X3X4.It is concluded from the model that when temperature,pH value,time and substrate concentration are: 35 ℃,6.8,40 min and 20μM respectively,the yield (Y) is the maximal (55.62 U/g) and consistent with the validate experiment result.
Chalcone isomerase activity Assay condition Orthogonal rotation combination design Optimization
Feng Xu Chenshan Xu Shuhan Cheng Shuiyuan Cheng
College of Horticulture and Gardening,Yangtze University,Jingzhou 434025,China; College of Horticult College of Horticulture Science and Engineering,Shandong Agricultural University,Taian 271018,China College of Horticulture and Gardening,Yangtze University,Jingzhou 434025,China
国际会议
山东泰安
英文
906-910
2008-07-25（万方平台首次上网日期，不代表论文的发表时间）