COMPARISON OF DIFFERENT ANTISERA IN ORDER TO DETECT PREGNANCY ASSOCIATED GLYCOPROTEIN (PAG) IN SWAMP BUFFALO
This study was conducted in order to test the ability of three different RIA to detect PAGs in swamp buffalo fluids. The three antisera were raised in rabbit immunized against purified bovine PAG67kDa (As497 or RIA 1), against caprine PAG55+62kDa(As706 or RIA 2) and buffalo PAG (As 859 or RIA 3). The RIA systems were taken with and without preincubation of unknown sample with antibody.In all assays, the tracer and the standard were prepared from a purified preparation of PAG 1. The sera were withdrawn from pregnant buffalo at different time of pregnancy. All the RIA systems were able to detect PAG from low concentrations. The dilutions of different samples of swamp buffalo were parallel with the standard. The aforementioned systems were shown suitable for PAG determination in swamp buffalo.
Swamp buffalo PAG RIA antibody
N. Van HANH Q.X. HUU N.T. UOC O. BARBATO J. SULON M.N. SOUSA J.F. BECKERS X.N. BUI
Laboratory of Embryo Technology-Institute of Biotechnology- Vietnamese Academy of Science and Techno Department of Biopathological Veterinary Science, Faculty of Veterinary Medicine, University of Peru Department of Physiology of Animal Reproduction, Faculty of Veterinary Medicine, University of Liege
国际会议
The 5th Annual Conference of the Asian Reproductive Biotechnology Society(第五届亚洲动物繁殖生物技术大会)
昆明
英文
110-114
2008-11-26（万方平台首次上网日期，不代表论文的发表时间）