Study of Regenerated Bud Culture from Explants of Lagerstroemia Indica Leaves

摘要：

. Lagerstroemia indica L is famous for its flowers especially in spring and summer. In our research, we chose new and old leaves of L. indica as explants. Based on the MS medium, we added different concentrations of auxin such as 2,4-D, 6-BA, IAA and NAA in order to study the L. indica tissue culture technology. By researching regenerated buds of L. indica and preliminary exploration, we built the asepsis propagation system and obtained the method of inducing calli and buds differentiation. At the same time, we found the high efficient approach of regenerated bud culture by choosing L. indica leaves as explants. Our experiment adopted two methods to induce buds differentiation. One was a two-step method which induced calli at first and then transferred calli into buds differentiation media. Another was that explants can produce buds directly on the medium. The results showed that puting leaves on the MS + 6-BA 2.0 mg/L + IAA 0.2mg/L medium can induce calli and produce buds, and such direct training division is the most efficient way.

关键词： Lagerstroemia indica regenerated bud culture explants of leaves calli.

作者: Xiuchun Dong Yan Shi Wei Guo Jungang Wang Jinhui Fan Huairui Shu

作者单位: College of Forestry, Shandong Agricultural University, Taian Shandong China, 271018 Forestry College of Zhejiang,Zhejiang China,311300 College of Horticulture Science and Engineering,Shandong Agricultural University,Taian Shandong Chin

会议类型: 国际会议

会议名称: The 1st International ELID-Grinding Conference(第一届镜面磨削技术国际会议)

会议地点: 长沙

会议语种:英文

页码: 911-915

在线出版日期: 2008-06-12（万方平台首次上网日期，不代表论文的发表时间）