Effect of Ginseng Polysaccharide on Enteric Mucosal Lymphocytes in Rats With Collagen Induced Arthritis

摘要：

Aim:To investigate the effects of ginseng polysaccharide on T-lymphocytes in enteric mucosal lymphocytes in collagen induced arthritis (CIA) in rats.Methods:The peyers patche lymphocyte (PPL),intraepithelial lymphocyte (IEL) and lamina propria lymphocyte (LPL) of SD rats were isolated.The PPL,IEL and LPL were checked with flow cytometry,and these lymphocytes were co-cultured with ginseng polysaccharide at different dosages.ELISA measured the levels of TNF-a and IFN-γ in supernatants.Results:The results showed that ginseng polysaccharide could increased the level of TNF-a and IFN-γ in the supernatant of PPL on normal rats,and it could lower the level of TNF-a while increase the IFN-γ level in the supematant of PPL on CIA rats.Ginseng polysaccharide could lower the levels of TNF-a and IFN-γ in the supernatant of LPL on normal rats.And it could lower the levels of TNF-a and IFN-γ in the supematant of both IEL and LPL on CIA rats.Conclusion:Ginseng polysaccharide could regulate the function of Lymphocytes in enteric mucosal immune system in CIA rats,and these indicate that Ginseng polysaccharide could be used in the treatment of autoimmune disease.

关键词： Ginseng polysaccharide collagen induced arthritis Lymphocyte Intestinal Mucosal Immunity

作者: Zhao Hong-yan Zhang Wan-dong LU Cheng LU Ai-ping

作者单位: Institute of Basic Theory,China Academy of Traditional Chinese Medicine,Beijing 100700 1st Affiliated Hospital of Anhui Traditional Chinese Medicine College,Hefei,Anhui 230031 Institute of Basic Research in Clinical Medicine,China Academy of Traditional Chinese Medicine,Beiji

会议类型: 国际会议

会议名称: 2008 International Conference on Ginseng(2008国际人参会议)

会议地点: 长春

会议语种:英文

页码: 293-303

在线出版日期: 2008-09-01（万方平台首次上网日期，不代表论文的发表时间）