Preliminary Study on Molecular Recognition of Pathogenic Vibrio Based on SELEX Techniques

摘要：

Based on the designment of the 76mer ssDNA library and choice of affinity determination method, a SELEX system to select the high affinity aptamers against Vibrio alginolyticus was preliminarily established and the high affinity aptamers were selected through 5 rounds of the SELEX.The study showed that the non-specific amplification in the replication of the ssDNA library was inhibited in the end phase of the SELEX. The high affinity aptamers that would be used for the molecular recognition of the microorganism were becoming more and more with the increasing SELEX rounds, though the affinity of ssDNA library decreased in some SELEX round. The affinity of the ssDNA library increased by 80% through only 5 rounds of the SELEX.

关键词： Vibrio alginolyticus SELEX ssDNA library Affinity

作者: LI Ting ZHENG Jiang

作者单位: Fisheries College of Jimei University, Key Laboratory of Science and Technology for Aquaculture and Fisheries College of Jimei University, Key Laboratory of Science and Technology for Aquaculture and

会议类型: 国际会议

会议名称: 2008 International Conference on IAEA(2008农业信息化、自动化与电气化国际会议)

会议地点: 镇江

会议语种:英文

页码: 624-628

在线出版日期: 2008-11-01（万方平台首次上网日期，不代表论文的发表时间）