Discrimination of Cocoon Sex Using Middle-Infrared Spectroscopy with the Aid of Chemometrics
Study on method of discriminating cocoon sex on basis of middle-infrared spectroscopy.Cocoon was cut to identify cocoon sex through examining its pupa, and male and female sex was set to values of 1 and 0 respectively. It were acquired that the attenuated total reflectance spectra of cocoon samples from wavenumber 4 000 cm-1 to 600 cm-1. After one odd spectrum was rejected through principal component cluster analysis, twenty-seven samples were selected randomly as calibration set and the other 13 were as test set. It was analyzed that the influence of Savitzky-Golay convolution smooth (window points was 3, 5, 7, 9, 11 and 13) and different principle components (1-10) on prediction accuracy. Establish PLS model and artificial neural networks model. Results indicated that 100 percent prediction accuracy for both calibration and prediction sets could be achieved when 3 principle components and 3 window points for Savitzky-Golay convolution smooth were adopted in PLS model, or 2 principle components and 5 window points for Savitzky-Golay convolution smooth. It indicated that MIR spectroscopy can be used for prediction of cocoon sex.
Cocoon sex Middle-Infrared Spectroscopy PLS Savitzky-Golay convolution smooth
YAN Hui CHEN Bin
School of Food and Biological Engineering, JiangSu University, P.R.China 212013 School of Biological School of Food and Biological Engineering, JiangSu University, P.R.China 212013
国际会议
2008 International Conference on IAEA(2008农业信息化、自动化与电气化国际会议)
镇江
英文
617-623
2008-11-01（万方平台首次上网日期，不代表论文的发表时间）