Study on Amplification Method of ssDNA Library in Detection of Pathogenic Vibrio by SELEX

摘要：

System evolution of ligands by exponential enrichment (SELEX) was applied to select aptamers that can recognize pathogenic microorganism Vibrio alginolyticus. Amplificaition of ssDNA library is the key step in this technique. The effects of asymmetric PCR method and simplified magnetic beads (SMB) method on the amplification of ssDNA library were comparatively studied in the present paper. The results show that the asymmetric PCR method has lower efficient amplification; the later purification process of ssDNA also makes it inconvenient and hard to manipulate. SMB method has higher efficient amplification for the ssDNA library. A new improved amplification method, SMB method for ssDNA library was developed by changing the purification step into neutralization step.

关键词： Vibrio Measurement SELEX ssDNA library Amplifiction

作者: ZHENG Jiang SU Yongquan

作者单位: College of Oceanography and Environmental Science, Xiamen University, Xiamen, China, 361005 Key Labo College of Oceanography and Environmental Science, Xiamen University, Xiamen, China, 361005

会议类型: 国际会议

会议名称: 2008 International Conference on IAEA(2008农业信息化、自动化与电气化国际会议)

会议地点: 镇江

会议语种:英文

页码: 164-168

在线出版日期: 2008-11-01（万方平台首次上网日期，不代表论文的发表时间）