Phase Shifts of Circadian Transcripts in Rat Suprachiasmatic Nucleus
We analyzed the phase shifts of oscillated transcripts relative to the timing of phase-reset stimuli. Oscillations in gene expression profiles were extracted using the fast Fourier transform and fitted by sine curve with random periods. The phase differences among multiple phase-reset conditions were analyzed to elucidate the mechanism of the core circadian clock. For the expression profiles, cultured cells of rat suprachiasmatic nucleus were measured by Aflimetrix GeneChip system. Folskolin stimulus was used as a phase-reset agent, causing irregular shift characteristic to oscillatory transcripts. The results suggest that the fluctuations of gene expressions in the core clock fall into two major categories and can be shifted by folskolin. Other clock related genes might adjust their oscillation by counter-steering each other in the feed-back regulation system.
Ryoko Morioka Masanori Arita Katsuhiko Sakamoto Soshi Kawaguchi Hajime Tei Katsuhisa Horimoto
Computational Biology Research Center, Advanced Industrial Science and Technology, Koto, Tokyo, Japa Computational Biology Research Center, Advanced Industrial Science and Technology, Koto, Tokyo, Japa Mitsubishi Kagaku Institute of Life Science, Machida, Tokyo, Japan
国际会议
The Second International Symposium(OSB08)(第二届国际优化及系统生物学学术会议)
云南丽江
英文
109-114
2008-10-31（万方平台首次上网日期，不代表论文的发表时间）