Identification of intracellular kinetic parameters in continuous bioconversion of glycerol by Klebsiella pneumoniae

摘要：

In this paper, we propose a hybrid nonlinear dynamical system to describe the concentration changes of extracellular and intracellular substances of glycerol bioconversion to 1,3-propanedol (1,3-PD) in microbial continuous cultures. It is proved that the solution to the system exists and is continuous with respect to kinetic parameters. Subsequently, a novel quantitative definition of biological robustness is investigated. We present a performance index based on experiment data of extracellular concentrations and biological robustness. Taking the proposed hybrid nonlinear dynamical system as a constraint, we establish an identification model to determine the most reasonable metabolic pathway and optimal kinetic parameters. The identifiability of the proposed model is also ascertained. This work provides theoretical basis for future numerical simulation and optimization computation.

关键词： Hybrid dynamical system Continuous culture Parameters identification Robustness analysis

作者: En-min Feng Chong-yang Liu Zhao-hua Gong Ya-qin Sun

作者单位: Department of Applied Mathematics Dalian University of Technology, Dalian 116024, Liaoning, China Department of Biotechnology Dalian University of Technology, Dalian 116012, Liaoning, China

会议类型: 国际会议

会议名称: The Second International Symposium(OSB08)(第二届国际优化及系统生物学学术会议)

会议地点: 云南丽江

会议语种:英文

页码: 101-108

在线出版日期: 2008-10-31（万方平台首次上网日期，不代表论文的发表时间）