Influence of carbonyl stress on rheological alterations of blood materials and decarbonylation effect of glutathione
The effects of various toxic carbonyis such as malondialdehyde (MDA), a secondary product of lipid peroxidation, and other aldehydes on rheological parameters and their relationship with aging-associated alterations were studied. Both MDA and glutaraldehyde (Glu) in different concentrations significantly increase viscosity, plastic viscosity and yield stress of human plasma and rythrocyte suspensions. MDA (20 mmol/L) reduces sharply the typical fluorescence of proteins (excitation 280 nm/emission 350 nm), and produces age pigment-like fluorescence with a strong emission peak at 460 nm when excites at 395 nm by only being incubated for some hours. In contrast, Glu decreases merely the fluorescence of proteins without producing age pigment-like fluorescence. These data suggest interestingly that the MDA-induced gradual protein cross linking seems to form from different mechanisms compared to the fast rheological changes of blood materials which may take place either in acute and chronic diseases or during aging. On the other hand, MDA induces various deleterious alterations of erythrocytes whereas glutathione (GSH) inhibits the MDA-related carbonyl stress in a concentration-dependent manner. The results indicate that carbonyl-amino reaction exists in the blood widely and GSH has the ability to interrupt or reverse this reaction in a certain way. It implies that carbonyl stress may be one of the important factors in blood stasis and suggests a theoretical and practical approach in anti-stresses and anti-aging.
blood viscosity carbonyl stress malondialdehyde cross linking decarbonylation effect glutathione
PENG Mi-jun(彭密军) CAI Jian-guang(蔡建光) HE Hong(贺洪) GONG Ping(龚萍) LI Guo-lin(李国林) TANG Ting(汤婷) ZHU Ze-rui(朱泽瑞) YIN Da-zhong(印大中)
Key laboratory of Protein Chemistry and Developmental Biology of Ministry of Education, Hunan Normal Key laboratory of Protein Chemistry and Developmental Biology of Ministry of Education, Hunan Normal
国际会议
长沙
英文
540-544
2008-09-28（万方平台首次上网日期，不代表论文的发表时间）