Droplet Microfluidic Technologies for the Real World

摘要：

We shall present a series of technologies allowing robust, high throughput applications of water droplets transported in an oil flow, acting as independent microreactors. These technologies address yet unsolved problems such as the world-to-chip interface, high yield single cell encapsulation, or the control of drop-to-drop contamination for demanding applications such as PCR. In particular, we present a fully automated system for performing continuous sampling, reagent mixing and Polymerase Chain Reaction (PCR) in microdroplets. Reproducible amplification, negligible cross contamination and low detection limits were demonstrated in continuous operation for several months at a yield of 3000 reactions per day. The system, which opens the route to strong reagents and labor savings in high throughput applications, was validated on the clinically relevant quantificatioti of progesterone receptor gene expression in human breast cancer cell lines. Other applications relevant to cell biology will also be presented in the conference.

关键词： Droplet microfluidics Automation Interface PCR Cell biology

作者: Max Chabert Guilllaume Colas Ghislaine Gavarin Jacques Goulpeau Jean-Louis Viovy

作者单位: Institut Curie UMR168.26, rue dUlm. 75005 Paris. France Institut Curie UMR168.26, rue dUlm. 75005 Paris. France Current address: CEMS, U. Minnesota, 421 Wa

会议类型: 国际会议

会议名称: The First Shenyang International Colloquium on Micrifluidics(首届沈阳国际微流控学术论坛)

会议地点: 沈阳

会议语种:英文

页码: 69-71

在线出版日期: 2007-10-21（万方平台首次上网日期，不代表论文的发表时间）