Squeezing-Sucking Driven Microfluidic PCR Device Based on Microfabricated PDMS Pneumatic Valves

摘要：

A squeezing-sucking driven microfluidic polymerase chain reaction (PCR) system was developed based on microfabricated polydimethylsiloxane (PDMS) pneumatic valves, and rapid cycling in a closed loop was achieved. When using 2.6μL reaction chambers, 1 second sufficed the transfer of the sample between two temperature zones. By using this system, a 200bp fragment from SEA DNA was successfully amplified within 23min.

关键词： PCR Microfluidics Pneumatic valve Squeezing-sucking driven

作者: Xin Wang Zhang-Run Xu Jian-Hua Wang Zhao-Lun Fang

作者单位: Research Center for Analytical Sciences, Northeastern University, Shenyang, China

会议类型: 国际会议

会议名称: The First Shenyang International Colloquium on Micrifluidics(首届沈阳国际微流控学术论坛)

会议地点: 沈阳

会议语种:英文

页码: 94-95

在线出版日期: 2007-10-21（万方平台首次上网日期，不代表论文的发表时间）