Simple Immobilization Method of Protein and Adhesive Cell on Functionalized Surface
This study presented simple surface modification for the immobilization of biomolecules such as proteins and cells in order to integrated with microfluidic system. Firstly, the fabrication of thin film composed of glycidyl methacrylate (GMA) that amine- attractive epoxide monomer was fabricated with photopolymerization. The orthogonal surface using simple micromolding in capillaries (MIMIC) was obtained. One region included polyethylene glycol (PEG) microstructures that prevent nonspecific binding of proteins and cells. The other region was the epoxide surface that promoted protein and cell immobilization. The orthogonal difference in surface functionality between the epoxide region and background PEG show strong possibility of simple patterning of biomolecules. In addition, the size, shape, height of pattern could be easily controlled by PDMS mold and spinning condition. It will be useful platform technology for the constrcution of organic material patterning, protein array, and cell.
Glycidyl methacrylate Surface modification Protein patterning Cell patterning
Hwan-Moon Song Bo-Yeol Kim Chang-Hyoung Choi Jae-Hoon Jung Chang-Soo Lee
Department of Chemical Engineering, Chungnam National University, 220 Gung-Dong, Yu-Seong Gu Daejeon, 305-764, Korea
国际会议
The First Shenyang International Colloquium on Micrifluidics(首届沈阳国际微流控学术论坛)
沈阳
英文
128-131
2007-10-21（万方平台首次上网日期，不代表论文的发表时间）