Cell Culture Array with a Concentration Gradient-Generating Microfluidic Structure
This paper introduced a novel cell culture array chip with a concentration gradient-generating microfluidic structure. The chip was fabricated with poly (dimethylsiloxane) (PDMS) by the soft lithography. The Rhodamine solution was used to test the generation of the concentration gradients. The 3T3 cell was perfused into the microchannels and trapped in the dam array for culture. The Fluo-4AM solution formed concentration gradients via the microfluidic structure and then stained the cells respectively. The result verified that the chip succeeded in both the cell culturing and the generation of gradients, which was possible to apply for the drug screening and the system biology study.
Microfluidic Gradient Cell culture PDMS Rhodamine Fluo-4AM
Hui Zhao Lei Wu Qiang Chen Qing-hui Jin Jian-Long Zhao
Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai
国际会议
The First Shenyang International Colloquium on Micrifluidics(首届沈阳国际微流控学术论坛)
沈阳
英文
135-138
2007-10-21（万方平台首次上网日期，不代表论文的发表时间）