Micromolded Agarose Gel Structure on Microchip for Sample Preconcentration

摘要：

A novel method for integrating a nanoporous membrane in microfluidic devices is introduced. The nanoporous agarose gel membrane is fabricated in-situ on PDMS microchips via micromolding techniques. The membrane is just a thin gel diaphragm perpendicularly embedded in the separation channel and close to the junction of the sample injection and separation channels, which enables electrokinetic concentration of DNA samples by a size exclusion mechanism prior to separation. This design allows a concentrated and well-defined sample plugs injected into the separation channel for sensitive high-resolution electrophoresis analysis. Experiments show that the DNA fragments can be concentrated up to nearly 1 order of magnitude greater than initial sample concentration within 10 sec.

关键词： Agarose gel Microfluidics Micromolding Sample preconcentration

作者: Qiang Chen Gang Li Qing-Hui Jin Jian-Long Zhao

作者单位: Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences Shanghai, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences Shanghai,

会议类型: 国际会议

会议名称: The First Shenyang International Colloquium on Micrifluidics(首届沈阳国际微流控学术论坛)

会议地点: 沈阳

会议语种:英文

页码: 139-142

在线出版日期: 2007-10-21（万方平台首次上网日期，不代表论文的发表时间）