Multi-Walled Carbon Nanotubes Coated Silica Gel and Its Application to Solid-Phase Extraction and Preconcentration of Protein Species
A new sorbent for solid phase extraction/preconcentration of proteins was prepared by coating multi-walled carbon nanotubes on silica gel spheres (MWNTs/SiO<,2>) using polyelectrolyte- assisted layer-by-layer (LBL) assembly technique. The adsorption behavior of cytochrome c onto the MWNTs/SiO<,2> spheres is in good agreement with the Langmuir adsorption model. The effects of pH, the concentration and composition of the eluent on the retention behavior of cytochrome c were investigated. A maximum sorption capacity for cytochrome c of 250 mg g<-1> was achieved. An enrichment factor of 30 was derived with a sample loading volume of 2.0ml. The micro-column packed with the MWNTs/SiO<,2> sphere facilitates high throughput attributed to its decreased flow impedance, which results in a high efficiency of protein separation and preconcentration.
Multi-walled carbon nanotubes Layer-by-layer assembly Silica gel Proteins Solid phase extraction
Zhuo Du Jian-Hua Wang
Research Center for Analytical Sciences, Northeastern University, Shenyang, China
国际会议
The First Shenyang International Colloquium on Micrifluidics(首届沈阳国际微流控学术论坛)
沈阳
英文
147-148
2007-10-21（万方平台首次上网日期，不代表论文的发表时间）