Whole Column Fluorescence Imaging on Microchip by Using Programmed Organic Light Emitting Diode Array as Spatial-Scanning Light Source and Single Photomultiplier Tube as Detector
A novel miniaturized, integrated whole-column imaging detection (WCID) system on microchip was presented. In this system, a program controlled organic light emitting diode (OLED) array was used as a spatial- scanning light source, to achieve imaging by the time sequence of the excited fluorescence. By this mechanism, photomultiplier tube (PMT) instead of charge coupled detector (CCD) can be applied to imaging, and lens, fibers or any mechanical components are not required either. The novel flat light source provides uniform excitation light without size limitation and outputs stronger power by pulse driving. The scanning mode greatly reduced the power consumption of the light source, which is valuable for a portable system. Meanwhile this novel simplified system has broader linear range, higher sensitivity and higher efficiency in data collection. Isoelectric focusing (IEF) of R-phycoerythrin (PE) and monitoring of the overall process with WCID were performed on this system.
Kang-Ning Ren Qiong-Lin Liang Bo Yao Guo-An Luo Li-Duo Wang Yu-Di Gao Yi-Ming Wang Yong Qiu
Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology Department of Chemistry, Tsingh Key Lab of Organic-Optoelectronics & Molecular Engineering of Ministry of Education Department of Ch Beijing Visionox Technology Co., Ltd, Beijing, China Key Lab of Organic-Optoelectronics & Molecular Engineering of Ministry of Education Department of Ch