Development of New Microfluidic Immunoassay Chips

摘要：

This paper reports a new platform for chip-based immunoassay using photopolymer and antibody- immobilized small-sized polystyrene beads (1μφ). Hydrogel pillars, which contain the antibody-immobilized polystyrene beads, in microchannels were fabricated by photolithography. By using this format, we realized (i) rapid analysis (a few minutes), (ii) easy handling (no need pump and valve), (iii) low-cost (disposable), (iv) small volume (1 droplets (0.25μL) of sample and reagents), and (v) high sensitivity.

关键词： Immunoassay Photopolymer Photopolymerization Hydrogel pillar

作者: Manabu Tokeshi Mai Ikami Noritada Kaji Yoshinobu Baba

作者单位: Department of Applied Chemistry, Nagoya University, Nagoya, Japan MEXT Innovative Research Center of Department of Applied Chemistry, Nagoya University, Nagoya, Japan Department of Applied Chemistry, Nagoya University, Nagoya, Japan MEXT Innovative Research Center of

会议类型: 国际会议

会议名称: The First Shenyang International Colloquium on Micrifluidics(首届沈阳国际微流控学术论坛)

会议地点: 沈阳

会议语种:英文

页码: 199-200

在线出版日期: 2007-10-21（万方平台首次上网日期，不代表论文的发表时间）