Quantum Dot-Encoded Microbeads: Synthesis and Application to Microchip Flow Cytometer for Diagnosis

摘要：

A series of quantum dot(QD)-encoded polystyrene microbeads is prepared. Compared with microbeads loaded with traditional organic dyes, as-synthesized fluorescence microbeads have better photostability. These microbeads could conjugate with protein by further surface modification, so they could be applied to such fields as biochemistry, molecular biology, cell biology, immunology. A home-made microfluidic system is designed and fabricated, and coupled with flow cytometry for QD-encoded microbeads analysis. The microbeads modified with biomolecules can be used for pathogen detection. A method for detecting epidemic hemorrhagic fever and new-castle disease based on such microbeads was developed. It was shown the set-up could be a good platform for detecting multiple disease pathogens, simultaneously.

关键词： Microfluidics Quantum dots Microbeads

作者: Ying Mu Rui Yang Wei Jin Yu-Xi Yan Lei Tao Qin-Han Jin

作者单位: Research Center for Analytical Instrumentation, College of Information Science and Engineering Zheji College of Chemistry, Jilin University, Changchun, 130023, P. R. China Research Center for Analytical Instrumentation, College of Information Science and Engineering Zheji Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University C Research Center for Analytical Instrumentation, College of Information Science and Engineering Zheji

会议类型: 国际会议

会议名称: The First Shenyang International Colloquium on Micrifluidics(首届沈阳国际微流控学术论坛)

会议地点: 沈阳

会议语种:英文

页码: 215-218

在线出版日期: 2007-10-21（万方平台首次上网日期，不代表论文的发表时间）