Study on Chemiluminescence Reaction of Dichlorofluorescein-Bovine Serum Albamin by Flow-Injection Method
The chemiluminescence (CL) characteristics of bovine serum albumin (BSA) labeled with dichlorofluorescein (DCF) has been studied by using flow injection method. The experimental conditions for the DCF-BSA- ClO-cetyltrimethylammonium bromide (CTAB) CL system were optimized. Based on the relations between CL intensity and BSA concentration; a new method has been established for the quantitative determination of BSA. Under the optimum conditions, the proposed method has a linear range of 0.01 to 20.0μg/ml, with a detection limit of 0.007μg/ml for BSA (3σ). The relative standard deviation of 1.0μg/ml BSA (n=8) is 1.4％. The method was applied to the determination of BSA in milk sample with satisfactory results.
Dichlorofluorescein Bovin serum albumin Chemiluminescence Flow injection
Chun-Bao Huang Kai Zhang Wen-Yue Yang Xiao-Man Mo Sheng-Fu Wang
School of Chemistry and Applied Chemistry, Huanggang Normal University, Huangzhou 438000, China Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecul
国际会议
The First Shenyang International Colloquium on Micrifluidics(首届沈阳国际微流控学术论坛)
沈阳
英文
227-230
2007-10-21（万方平台首次上网日期，不代表论文的发表时间）